An indirect ELISA was established with expressed 3a protein of FMDV as coating antigen and was experimentally used to detect FMDV infection sera, immune sera and other disease sera.

用表达的口蹄疫3a蛋白作为包被抗原，建立了间接elisa试验，对口蹄疫病毒感染血清、免疫血清和其它非口蹄疫病毒血清进行了检测研究。

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The immune rabbit sera were used for passive immunity test in mice.

并以家兔免疫血清在小鼠进行被动免疫保护实验。

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The method was proved to be very sensitive, specific and repetitive by detecting different sera samples from clinical and immune ducks, which can diagnose the disease rapidly.

经对不同发病日龄和免疫油苗后雏鸭的血清检测，证明该检测方法有很高的敏感性、特异性和重复性，可作为快速诊断该病的一种可靠方法。

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